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Tumor necrosis factor-(TNF-α): An important pro-inflammatory cytokine

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Tumor Necrosis Factor-alpha (TNF-α) is a cytokine that acts as an amplifier of inflammation in almost all acute inflammatory responses. TNF-α blockade has been utilized to treat inflammatory disorders such as rheumatoid arthritis all over the world. Clinical trials are also being conducted to see if TNF-α may be used to treat cancer and cardiovascular problems.

TNF-α is a member of the TNF superfamily. Extracellular proteolytic cleavage can unleash proteins from the cell membrane, allowing them to function as cytokines. TNF-αtrimer functions by attaching to the trimeric receptors TNFR-1 (CD120a) or TNFR-2(CD120b). Members of the TNF receptor superfamily include FAS, CD40, CD27 and RANK. TNFR1 signaling is both pro-inflammatory and pro-apoptotic.

See More Reagents for TNF-α Research

NF-κB, mitogen-activated protein kinases, and death signaling are all triggered by it.Other pro-inflammatory cytokines (IL-1, IL-6, GM-CSF, and so forth) may then be activated. TNFR2 signaling, on the other hand, is anti-inflammatory and stimulates cell proliferation. It triggers the conventional NF-κB and PI3K/Akt signaling pathways.

To support in the research of this cytokine, Sino Biological has created a panel of high-quality recombinant TNF-α and receptor proteins.

Recombinant TNF-α cytokines and receptors

Sino Biological has created bioactive recombinant TNF-αcytokines from a variety of species, including humans, mice, cynomolgus, rats, ferrets and canines.

Featured TNF-α protein

Human TNF-α protein: 10602-HNAE

  • HPLC-verified
  • Low-endotoxin
  • High Purity
  • Tag-free
Cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

Cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. Image Credit: Sino Biological Inc.

Batch-to-batch Consistency.

Batch-to-batch Consistency. Image Credit: Sino Biological Inc.

greater 95% as determined by SEC-HPLC.

>95% as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

Other recombinant TNF-α cytokines

Table 1. Source: Sino Biological Inc.

Cat# Species Expressed Host Tag Purity Activity
10602-H01H Human HEK293 Cells N-hFc >90% Active
10602-HNAE-B Human E. coli Native >90%  
50349-MNAE Mouse E. coli Native >98% Active
50349-MNAE-B Mouse E. coli Native >95%  
60002-FNAE Ferret E. coli Native >95% Active
70003-DNAE Canine E. coli Native >96% Active
80045-RNAE Rat E. coli Native >93% Active
90018-CNAE Cynomolgus, Rhesus E. coli Native >90% Active
90330-CNAE Cynomolgus E. coli Native >95% Active
90330-CNAE-B Cynomolgus E. coli Native >95%  

 

Recombinant TNF-α receptors

Table 2. Source: Sino Biological Inc.

Cat# Receptor Species Expressed Host Purity Tag Activity
10872-H02H TNFR-1 Human HEK293 Cells >95% C-hFc Active
10872-H03H TNFR-1 Human HEK293 Cells >90% C-hFc & His Active
10872-H08H TNFR-1 Human HEK293 Cells >92% C-His Active
50496-M02H TNFR-1 Mouse HEK293 Cells >90% C-hFc Active
50496-M08H TNFR-1 Mouse HEK293 Cells >96% C-His Active
80181-R02H TNFR-1 Rat HEK293 Cells >90% C-hFc Active
80181-R08H TNFR-1 Rat HEK293 Cells >95% C-His  
10417-H03H TNFR-2 Human HEK293 Cells >95% C-hFc & His Active
10417-H08H TNFR-2 Human HEK293 Cells >97% C-His Active
10417-H08H-B TNFR-2 Human HEK293 Cells >95% C-His  
10417-H08H1 TNFR-2 Human HEK293 Cells >95% C-His Active
90102-C02H TNFR-2 Cynomolgus, Rhesus HEK293 Cells >95% C-hFc Active
90102-C08H TNFR-2 Cynomolgus, Rhesus HEK293 Cells >95% C-His  
50128-M02H TNFR-2 Mouse HEK293 Cells >95% C-hFc Active
50128-M08H TNFR-2 Mouse HEK293 Cells >97% C-His Active

 

Other related proteins

  • RANK
  • CD40
  • FAS
  • CD27

Antibodies for TNF-α cytokines and receptors

  • TNFR-1
  • TNFR-2
  • TNF-α