Recombinant protein expression

Sino Biological is a global leader in recombinant technology and manufactures exceedingly high-quality and hard-to-express proteins.

With the availability of proprietary expression platforms, high-throughput and scale-up capacities, and more than 15 years of experience, Sino Biological has successfully developed targets of interest from gene synthesis to protein characterization, regardless of a project's complexity.

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Why choose Sino Biological?

Proprietary technologies

• Proprietary expression vectors, culture media, transfection reagents, etc.

Fast turnaround

• 12 Days: from gene synthesis to 100 mg eukaryotic protein production

High-throughput and Scale-up

• 1,000+ proteins available for every batch
• Flexible production scales: μg–kg level
• 80+ bioreactors: 2 to 1500 L

Multiple expression systems

• HEK293/CHO for stable and transient expression
• Cell-free system available
• FucoFree HEK293/CHO to generate afucosylated proteins
• E. coli
• Baculovirus-insect cell

Customized QC options

• SDS-PAGE, HPLC-SEC, UV, Endotoxin Test, ELISA, MS, Activity, etc.

Case study

Generating a protein with correct oligomeric status

• Active format: homo-dimmer
• Expression system: HEK293 cells
• Results: >95% dimerization and enhanced activity
• Strategy: initiating a molecular chaperone to streamline protein assembly
• Challenge: primarily exists as a monomer even following incubation → Low activity

Dimmer content

Image Credit: Sino Biological Inc.

Activity

Source: Sino Biological Inc.

Purity

Image Credit: Sino Biological Inc.