Phospholipase B Domain Containing 2 (PLBL2) is a protein-coding gene found on the human chromosome 12q24.13.
PLBL2 is also referred to as P76. A 65472 Da protein comprising 589 amino acids is encoded by the human PLBL2 gene. The PLBL2 protein is universally expressed in various tissues, including ovary and adrenal tissues.
While PLBL2 is associated with hydrolase activity, PLBD1 is a crucial paralog of the PLBL2 gene. PLBL2 is linked to certain diseases, such as Gallbladder Papillomatosis and Gm1-Gangliosidosis, Type Iii.
PLBL2/PLBD2 Protein, Chinese hamster, Recombinant (His Tag): Product Information
Purity
More than 95% as defined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg protein as defined by the LAL technique.
Activity
Test is ongoing
Protein construction
A DNA sequence, which encodes the Chinese hamster PLBD2 (G3I6T1-1) (Leu38-Asp585), was expressed at the C-terminus using a polyhistidine tag.
Expressed host
CHO cells
Species
Chinese hamster
Predicted N terminal
Leu 38
Molecule mass
The recombinant Chinese hamster PLBL2 contains a total of 559 amino acids and estimates a molecular mass of 63.3 kDa. Under reducing conditions, the recombinant Chinese hamster PLBD2 migrates as an approximately35.9, 43.8, 65.3 KDa band in SDS-PAGE.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Customers can contact Sino Biological for any concerns or unique needs. Generally, 0.01% Tween80 and 5% to 8% trehalose and mannitol are added as protectants prior to lyophilization. Customers can refer to the specific buffer data provided in the hard copy of CoA.
Shipping
Recombinant proteins are generally delivered as lyophilized powder that is shipped at ambient temperatures. Large recombinant protein packages are delivered as frozen liquid and are shipped with blue ice unless specified by customers.
Stability and storage
Specimens remain stable for up to one year from the date of receipt at −20℃ to −80℃. These should be stored under sterile conditions at −20℃ to −80℃. Users are advised to aliquot the protein for optimal storage. Repeated freeze-thaw cycles should be avoided.
Reconstitution
The products are delivered with a hard copy of COA containing reconstitution instruction. Customers can refer to it for thorough information.
PLBL2/PLBD2 protein, Chinese hamster, recombinant (His-tag): Image
Image Credit: Sino Biological Inc.
Background information of PLBL2/PLBD2
The PLBL2 gene localizes to the lysosome since its absence may lead to an unrecognized yet serious lysosomal storage disease. Both PLBD1 and PLBL2 are semi-orphans, which means they are likely to be the phospholipases of B class but with vague physiological substrates and thus functionalities.
The PLBD1 and PLBL2 genes represent a small gene family (sequence homology class) within the vertebrate species although one that manifests extended in some early diverging eukaryotes.
The PLBL2 gene poses a unique difficulty in that a series of post-translational steps are evidently required for its activation. Without these steps, promising substrates can seldom be tested.
Such post-translational steps involve mannosylation suitable for the lysosome targeting receptor, the removal of the signal peptide, and self-catalytic proteolytic activation to expose the binding site of the substrate as this turns out to be appropriate.
Full name
Phospholipase B domain containing 2
References
- Morgan CP. et al., 2004, Biochem J. 382 (2): 441-9.
- Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
- Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.