NKG2 ligands for immune checkpoint modulator research

The NKG2 (CD159) family of proteins includes NKG2D and NKG2A. The NKG2 proteins are significantly expressed on cytotoxic immune cells and form new appealing targets for developing immune checkpoint modulators.

The NKG2A is known as an inhibitor receptor and builds a heterodimer with CD94 (KLRD1). CD94 or NKG2A mixture reacts with the non-classical MHC class I molecule HLA-E on target cells.

This interaction prevents the triggering of T cells and NK cells through the tyrosine phosphates SHP-1 or SHP-2 signaling cascades.

Cancer cells use CD94/NKG2A-HLA-E, similar to how they use other immune checkpoints like PD-1/PD-L1, to evade the immune system.

NKG2D acts as an activating receptor. The activation of NKG2D by its ligands — such as MICA, MICB and ULBPs (ULBP1, ULBP2, ULBP3, ULBP4, ULBP5, ULBP6) — in human and H60 (a–c), RAE(α–ε) and MULT1 families in mice is a key antitumor signaling axis.

Using adaptor molecules, such as DAP10 and DAP12, the NKG2D protein transmits its signal transduction. By contrast, in humans, the NKG2D interacts mainly with DAP10.

In mice, NKG2D is also capable of controlling cytokine secretion (for example, IL-10, IL-9, IL-13). It also performs a crucial role in the fight against virus infection.

NKG2D/NKG2D ligands

Sino Biological has created highly active NKG2d and NKG2D ligands proteins from several species to support the research on NKG2Dor NKG2D ligand biology.

Binding activity validated by ELISA assay

Ligands bind with NKG2D (Cat#: 10575-H07B)

Human ULBP1 (Cat#: 10679-H03H). Image Credit: Sino Biological Inc.

Human ULBP6 (Cat#: 15759-H02H). Image Credit: Sino Biological Inc.

NKG2D binds with MICA (Cat#: 12302-H08H)

Human NKG2D (Cat#: 10575-H01S). Image Credit: Sino Biological Inc.

Recombinant NKG2D

Table 1. Source: Sino Biological Inc.

.	.	.	.
Cat#: 10575-H01S   Tag: Fc Purity: 95% (SDS-PAGE) Activity: Validated	Cat#: 10575-H07B   Tag: His Purity: 90% (SDS-PAGE) Activity: Validated	Cat#: 57340-M01B   Tag: Fc Purity: 90% (SDS-PAGE) Activity: Testing in progress	Cat#: 90164-C07B   Tag: His Purity: 95% (SDS-PAGE) Activity: Testing in progress

Note: H: Human; M: Mouse; Rh: Rhesus

Recombinant NKG2D ligands

Table 2. Source: Sino Biological Inc.

MICA
Human MICA (Cat#: HPLC-12302-H08H) >95% as determined by SEC-HPLC	12302-H02H   Tag: Fc Purity: 90% (SDS-PAGE) Activity: Testing in progress	12302-H08H   Tag: His Purity: 97% (SDS-PAGE) Activity: Validated
	12302-H08H1   Tag: His Purity: 95% (SDS-PAGE) Activity: Validated	90924-C08H   Tag: His Purity: 95% (SDS-PAGE) Activity: Testing in progress
MICB
10759-H03H Tag: Fc & His Purity: 98% (SDS-PAGE) Activity: Validated
10759-H08H Tag: His Purity: 98% (SDS-PAGE) Activity: Validated

Note: H: Human; Cy: Cynomolgus

NKG2A/NKG2A ligands

Sino Biological has developed NKG2A/NKG2A ligand proteins from several species.

Table 3. Source: Sino Biological Inc.

NKG2 related antibodies

Sino Biological has produced a detailed panel of antibodies that target NKG2A, NKG2D, NKG2E, MICA, MICB, ULBP1, ULBP2, which are capable of finding application in FCM, ELISA, WB, IF, IHC, etc. ELISA pair set and Human MICB ELISA kit are also available with the package.

Anti-NKG2A antibodies

Table 4. Source: Sino Biological Inc.

Anti-NKG2D antibodies

Table 5. Source: Sino Biological Inc.

Video credit: Sino Biological