Improve assay sensitivity and specificity with Locked Nucleic Acid oligonucleotides (LNA oligos) from LGC Biosearch Technologies. LNAs are RNA bases in which the ribose is “locked” with a methylene bridge, fixing the base in the C3’-endo conformation.
Locked nucleic acid
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| Image Credit: LGC Biosearch Technologies |
Benefits of LNA Probes
LNAs enable a shorter probe design, supporting the assay design for:
- Improved assay sensitivity
- Higher quenching efficiency (lower background)
- Greater signal-to-noise ratio
- Increased hybridization specificity
- Targeting AT-rich sequences
- Better mismatch discrimination in SNP genotyping assays
Order dual-labeled LNA Probes
LNAs for commercial use
You can maximize assay performance by harnessing the combined power of LNA technology and best-in-class BHQ quenchers. The Biosearch Technologies team is ready to help you optimize your assay. Provide the manufacturing team with your precise instructions using this Excel template today.
Additional advantages:
- Access LNA probes for multiplexing with a full suite of fluorophores.
- Insert up to seven LNA bases in the probe sequence.
- Purified via DUAL-HPLC and sequence verified via uHPLC and MS.
Save time and resources with pre-mixed LNA probes and primers
Ordering LNA probe and primer pairs as a ValuMix assay simplifies the assay setup process.
You can supply the specifications, including the probe-to-primer ratio, for gene expression and qPCR assays, and LGC sends you a unique, dual-labeled LNA probe and primer pair in a single tube.
LGC Biosearch Technologies provides a ValuMix assay for SNP genotyping applications that consists of two custom LNA probes and a primer pair delivered in exact quantities.
Customize your LNA oligo with an extensive menu of modifications
Take advantage of LNA-enhanced oligos, for example with your primers for added specificity in SYBR Green Assays. LGC Biosearch Technologies can synthesize custom oligos with internal LNA modifications. Simply add a "+" sign in front of the base to designate the LNA-base position (+A, +T, +C, +G) and insert up to 20 LNA bases.