Induced pluripotent stem cells (iPSCs) are a hopeful alternative for analyzing antigen expression in vitro and developing novel cellular therapies. iPSCs have been derived from differentiated adult somatic cells that are reprogrammed into an embryonic-like pluripotent state by making use of various transcription factors. They are capable of distinguishing into any kind of cell lineage.
With their limitless capacity for self-renewal and differentiation, such iPSCs hold enormous abilities in regenerative medicine, drug testing, disease modeling, and innovative cell or gene therapies.
The extensive selection of cytokines and growth factors, such as GM-CSF, DLL4, Activin A, NOG, VEGF, TNF-α, IL-2, FGF, IL1β, and EGF, are necessary components for distinguishing human iPSCs into several cell lineages. The applications of cytokines in iPSC studies are displayed in Table 1.
Table 1. List of cytokines and the applications in iPSC research. Source: Sino Biological Inc.
| Essential cytokines in iPSC research |
Application in research |
| GM-CSF, IL-3, M-CSF |
iPSC-derived monocytes and macrophages |
| DLL-4, IL-2, BMP4, TGF-β, IL33, IL10 |
iPSC-derived regulatory T cells (Tregs) |
| Activin A, BMP-4, MRAP, FGF2 |
iPSC-derived nephron progenitors |
| NOG, TNF-α, BMP-2, TGF-β1, FGF2 |
iPSC-derived mesenchymal stem cells (MSCs) |
| FGF10, BMP-4, TGF-β, FGF2 |
iPSC-derived lung epithelial cell lineages |
| VEGF121, FGF2, Ang-1, TNF-α |
iPSC-derived endothelial cells |
| EGF, OSM, HGF |
iPSC-derived hepatocytes |
| IL-1ββ, TPO, SCF |
iPSC-derived megakaryocytes |
Being a leading global supplier of high-quality recombinant cytokines, Sino Biological aids in quickly developing stem cell research.
Figure 1. Differentiation of induced pluripotent stem cells (iPSCs). Image Credit: Sino Biological Inc.
Featured recombinant iPSC cytokines
Sino Biological provides an extensive collection of stem cell-related growth factors and related protein reagents to iPSC research.
- High purity
- Activity-validated
- Cited by researchers
- HPLC-verified
Human DLL4 protein (Cat#: 10171-H02H) 9 citations
Immobilized mouse NOTCH1 (Cat: 50654-M08B) can bind human DLL4. Image Credit: Sino Biological Inc.
Human GM-CSF protein (Cat#: 10015-HNAH) 2 citations
Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.
Human Activin A protein (Cat#: 10429-HNAH) 5 citations
Ability to inhibit proliferation of MPC-11 cells. Image Credit: Sino Biological Inc.
Human FGF10 protein (Cat#: 10573-HNAE) 3 citations
Cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. Image Credit: Sino Biological Inc.
Mouse NOG protein (Cat#: 50688-M02H) 6 citations
Ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. Image Credit: Sino Biological Inc.
Human EGF protein (Cat#: 10605-HNAE) 57 citations
Cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. Image Credit: Sino Biological Inc.
More recombinant iPSC cytokines (Partial)
Table 2. Source: Sino Biological Inc.
| Cat# |
Species |
Proteins |
Expression Host |
Purity |
Activity |
Tag |
11792-H08H
6 Citations |
Human |
M-CSF/CSF1 |
HEK293 Cells |
> 95 % |
Active |
His |
51112-MNAH
11 Citations |
Mouse |
M-CSF/CSF1 |
HEK293 Cells |
> 95 % |
Active |
Native |
10015-H01H
4 Citations |
Human |
GM-CSF/CSF2 |
HEK293 Cells |
> 97% |
Active |
hFc |
51048-MNAH
5 Citations |
Mouse |
GM-CSF/CSF2 |
HEK293 Cells |
> 95 % |
Active |
Native |
| 80020-R01H |
Rat |
GM-CSF/CSF2 |
HEK293 Cells |
> 95 % |
Active |
hFc |
| 50659-M08H |
Mouse |
Activin A |
HEK293 Cells |
> 88 % |
Active |
His |
10267-H02H
3 Citations |
Human |
NOG/Noggin |
HEK293 Cells |
> 95 % |
Active |
hFc |
| 10267-HNAH |
Human |
NOG/Noggin |
HEK293 Cells |
> 95 % |
Active |
Native |
10171-HCCH
9 Citations |
Human |
DLL4 |
HEK293 Cells |
≥ 90 %
(SEC-HPLC) |
Active |
Native |
50640-M08H
1 Citation |
Mouse |
DLL4 |
HEK293 Cells |
> 96 % |
Active |
His |
90882-C08H
3 Citations |
Rhesus |
DLL4 |
HEK293 Cells |
> 95 % |
Active |
His |
10602-HNAE
30 Citations |
Human |
TNF-α |
E. coli |
≥ 95 %
(SEC-HPLC) |
Active |
Native |
50349-MNAE
15 Citations |
Mouse |
TNF-α |
E. coli |
> 98 % |
Active |
Native |
| 60002-FNAE |
Ferret |
TNF-α |
E. coli |
> 95 % |
Active |
Native |
70003-DNAE
1 Citation |
Canine |
TNF-α |
E. coli |
> 96 % |
Active |
Native |
80045-RNAE
6 Citations |
Rat |
TNF-α |
E. coli |
> 93 % |
Active |
Native |
| 90330-CNAE-B |
Cynomolgus |
TNF-α |
E. coli |
> 95 % |
Active |
Native |
10804-H08H
16 Citations |
Human |
TGF-β |
HEK293 Cells |
> 95 % |
Active |
His |
50698-M08H
6 Citations |
Mouse |
TGF-β |
HEK293 Cells |
> 95 % |
|
His |
80116-R08H
4 Citations |
Rat |
TGF-β |
HEK293 Cells |
> 90 % |
|
His |
90294-C08H
5 Citations |
Cynomolgus |
TGF-β |
HEK293 Cells |
> 95 % |
Active |
His |
10139-HNAE
13 Citations |
Human |
IL-1β |
E. coli |
≥ 95 %
(SEC-HPLC) |
Active |
Native |
50101-MNAE
14 Citations |
Mouse |
IL-1β |
E. coli |
> 97 % |
Active |
Native |
| 70018-DNAE |
Canine |
IL-1β |
E. coli |
> 96 % |
Active |
Native |
80023-RNAE
4 Citations |
Rat |
IL-1β |
E. coli |
> 98 % |
Active |
Native |
11848-H08H
2 Citations |
Human |
IL2 |
HEK293 Cells |
> 95 % |
Active |
His |
11848-HNAH1-E
1 Citation |
Human |
IL2 |
HEK293 Cells |
> 95 %
(SEC-HPLC) |
Active |
Native |
| 11848-HNAE2 |
Human |
IL2 |
E. coli |
≥ 95 %
(SEC-HPLC) |
Active |
Native |
51061-MNAE
8 Citations |
Mouse |
IL2 |
E. coli |
> 92 % |
Active |
Native |
70014-DNAE
2 Citations |
Canine |
IL2 |
E. coli |
> 95 % |
Active |
Native |
11858-HNAE
1 Citation |
Human |
IL3 |
E. coli |
> 95 % |
Active |
Native |
51066-MNAH
1 Citation |
Mouse |
IL3 |
HEK293 Cells |
> 95 % |
Active |
Native |
80235-R08B
1 Citation |
Rat |
IL3 |
Baculovirus-Insect Cells |
> 95 % |
Active |
His |
10947-H07H
6 Citations |
Human |
IL10 |
HEK293 Cells |
> 85 % |
Active |
His |
| 10947-HNAE |
Human |
IL10 |
E. coli |
> 95 % |
Active |
Native |
50245-MNAE
7 Citations |
Mouse |
IL10 |
E. coli |
> 95 % |
Active |
Native |
10368-HNAE
11 Citations |
Human |
IL33 |
E. coli |
> 95 % |
Active |
Native |
70005-DNAE
5 Citations |
Canine |
IL33 |
E. coli |
> 97 % |
Active |
Native |
10426-HNAE
10 Citations |
Human, Mouse, Rat, Rhesus, Canine |
BMP2 |
E. coli |
> 95 % |
Active |
Native |
63003-ZNAE
3 Citations |
Danio rerio |
BMP2 |
E. coli |
> 95 % |
Active |
Native |
50439-M15H
7 Citations |
Mouse |
BMP4 |
HEK293 Cells |
> 92 % |
|
rFc |
10605-H01H
40 Citations |
Human |
EGF |
HEK293 Cells |
> 90 % |
Active |
hFc |
10605-HNAE
57 Citations |
Human |
EGF |
E. coli |
≥ 95 %
(SEC-HPLC) |
Active |
Native |
50482-M01H
3 Citations |
Mouse |
EGF |
HEK293 Cells |
> 95 % |
Active |
hFc |
70013-D07E
1 Citation |
Canine |
EGF |
E. coli |
> 98 % |
Active |
His |
80446-RNAE
2 Citations |
Rat |
EGF |
E. coli |
> 95 % |
Active |
Native |
10014-HNAE
44 Citations |
Human |
FGF2 |
E. coli |
> 95 % |
Active |
Native |
50037-M07E
5 Citations |
Mouse |
FGF2 |
E. coli |
> 95 % |
Active |
His |
10573-HNAE
3 Citations |
Human |
FGF10 |
E. coli |
> 95 % |
Active |
Native |
10463-HNAS
16 Citations |
Human |
HGF |
CHO Stable Cells |
≥ 95 %
(SEC-HPLC) |
Active |
Native |
50038-MNAH
3 Citations |
Mouse |
HGF |
HEK293 Cells |
> 95 % |
Active |
Native |
| 70063-DNAH |
Canine |
HGF |
HEK293 Cells |
> 95 % |
Active |
Native |
80429-RNAH
2 Citations |
Rat |
HGF |
HEK293 Cells |
> 85 % |
Active |
Native |
| 90286-CNAH |
Cynomolgus |
HGF |
HEK293 Cells |
> 95 % |
Active |
Native |
11066-HNAH
8 Citations |
Human, Cynomolgus |
VEGF165 |
HEK293 Cells |
> 90 %
(SEC-HPLC) |
Active |
Native |
11066-H27H-B
13 Citations |
Human |
VEGF165 |
HEK293 Cells |
> 95 % |
Active |
His & AVI |
63004-ZNAB
7 Citations |
Danio rerio |
VEGF165 |
Baculovirus-Insect Cells |
> 90 % |
Active |
Native |
10008-HNAH
1 Citation |
Human |
VEGF121 |
HEK293 Cells |
> 95 %
(SEC-HPLC) |
Active |
Native |
10009-HNAB
2 Citations |
Human |
VEGF183 |
Baculovirus-Insect Cells |
> 95 % |
Active |
Native |
| 29714-HNAH |
Human |
VEGF110 |
HEK293 Cells |
> 95 % |
Active |
Native |
50159-MNAB
7 Citations |
Mouse |
VEGF164 |
Baculovirus-Insect Cells |
> 90 % |
Active |
Native |
80006-RNAB
2 Citations |
Rat |
VEGF164 |
Baculovirus-Insect Cells |
> 86 % |
Active |
Native |
| 13667-H02H1 |
Human |
Angiopoietin-1 |
HEK293 Cells |
> 85 % |
|
hFc |
| 50300-M07H |
Mouse |
Angiopoietin-1 |
HEK293 Cells |
> 95 % |
|
His |
| 90025-C07H |
Cynomolgus |
Angiopoietin-1 |
HEK293 Cells |
> 95 % |
|
His |
| 15444-H01H |
Human |
MRAP |
HEK293 Cells |
> 90 % |
|
hFc |
10452-HNAH
2 Citations |
Human |
OSM |
HEK293 Cells |
> 95 % |
Active |
Native |
50112-M08H
3 Citations |
Mouse |
OSM |
HEK293 Cells |
> 95 % |
|
His |
10451-H08B
2 Citations |
Human |
SCF |
Baculovirus-Insect Cells |
> 92 % |
Active |
His |
| 50487-M08B |
Mouse |
SCF |
Baculovirus-Insect Cells |
> 95 % |
Active |
His |
| 10381-H07H |
Human |
Thrombopoietin |
HEK293 Cells |
> 90 % |
Active |
His |
10381-H08C
1 Citation |
Human |
Thrombopoietin |
CHO Stable Cells |
> 85 % |
Active |
His |
| 50146-M08H |
Mouse |
Thrombopoietin |
HEK293 Cells |
> 90 % |
Active |
His |
Featured antibodies for stem cell research
High-quality antibodies are crucial in the characterization of iPSC cell differentiation. Sino Biological offers high-quality antibodies for stem cell markers and various lineage markers with applications in ELISA, IHC-IP, FCM, and IF or ICC.
Differentiated cell type: Monocytes and macrophages
Anti-CD45 antibody (Cat#: 102001-T08) 1 citation
Type: Rabbit PAb
Ig Type: Rabbit IgG
Applications: IHC-P
Immunochemical staining of mouse Ptprc in mouse brain. Image Credit: Sino Biological Inc.
Anti-CD14 antibody (Cat#: 10073-R002) 1 citation
Type: Rabbit Mab
Ig Type: Rabbit IgG
Applications: FCM
Flow cytometric analysis of CD14 expression on human whole blood monocytes. Image Credit: Sino Biological Inc.
Differentiated cell type: Nephron progenitors
Anti-E-cadherin antibody (Cat#: 10204-MM04) 7 citations
Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: ELISA, ICC/IF
Immunofluorescence staining of human E-cadherin in MCF-7 cells. Image Credit: Sino Biological Inc.
Anti-CD31 antibody (Cat#: 10148-MM13) 1 citation
Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: FCM, ICC/IF
Immunofluorescence staining of Human CD31 in HUVEC cells. Image Credit: Sino Biological Inc.
Differentiated cell type: Regulatory T cells
Anti-CD4 antibody (Cat#: 60003-MM02-A) 3 citations
Type: Mouse Mab
Ig Type: Mouse IgG1
Applications: FCM
Analysis of CD4 expression on spleen lymphocytes. Image Credit: Sino Biological Inc.
Anti-CD25/IL2R alpha antibody (Cat#: 50292-R049-C) 1 citation
Type: Rabbit Mab
Ig Type: Rabbit IgG
Applications: FCM
Flow cytometric analysis of mouse IL2RA(CD25) expression on mouse splenocytes. Image Credit: Sino Biological Inc.
Other related molecules