Interferon gamma (IFN-γ) is a cytokine that induces a series of immune responses against viruses and tumors. The pro-apoptotic, anti-tumor and anti-proliferative actions of the cells are activated when IFN-γ binds to the IFN-γ receptor, which is made up of IFNGR1 and IFNGR2.
IFN-γ expression that is erroneous encourages the growth of tumors. IFN-γ has thus become recognized as a major contributor to the immunogenicity of malignancy.
The CD4+ T helper cells and CD8+ cytotoxic T cells are the effector T cells that release IFN-γ. Natural killer cells, natural killer T cells, and B cells are additional immune cells that secrete IFN-γ.
IFN-γ is stimulated by interleukins such IL-12, IL-15, IL-18, and IL-21. These interleukins’ stimulatory effects also result from intracellular signaling of transcription factors such as AP-1, JAK/STAT, and T-bet.
In PD-1/PD-L1 immune checkpoint inhibitor cancer treatment, IFN-γ is a prognostic marker. The production of IFN-γ is associated with an improved response to immune checkpoint blockade (ICB) therapy in triple-negative breast cancer, and is considered to be an emerging high-potential adjuvant therapy target.
To facilitate the study of IFN- in cancer immunity, Sino Biological has produced a panel of recombinant IFN-γ protein and receptor reagents.
Featured IFN-γ and Receptor Proteins
Human IFN-γ Protein Cat#: 11725-HNAE
- Tag-Free
- HPLC-verified
- High Activity
- High-Purity
Purity: ≥ 95% as determined by SDS-PAGE & HPLC. Image Credit: Sino Biological Inc.
Anti-viral assays using WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Human IFNGR1 Protein Cat#: 10338-H08H
Ability to inhibit rhIFN-γ mediated protection of WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Mouse IFN-γ Protein Cat#: 50709-MNAH
Immobilized Mouse Interferon Gamma can bind Mouse IFNGR1 (Cat: 50705-M02H). Image Credit: Sino Biological Inc.
Antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Human IFNGR2 Protein Cat#: 50665-M08H
Purity: > 97 % as determined by SDS-PAGE. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Image Credit: Sino Biological Inc.
Product list of Recombinant IFN-γ & Receptors
Table 1. Source: Sino Biological Inc.
| Cat# |
Molecule |
Species |
Expressed Host |
Purity |
Activity |
Tag |
| 11725-HNAE |
IFNG |
Human |
E. coli |
≥95 % (by SEC-HPLC) |
Active |
Native |
| 11725-HNAS |
Human |
CHO Stable Cells |
>92% |
Active |
Native |
| 50709-M02H |
Mouse |
HEK293 Cells |
>92% |
Active |
C-hFc |
| 50709-M08H |
Mouse |
HEK293 Cells |
>75% |
Active |
C-His |
| 50709-MNAH |
Mouse |
HEK293 Cells |
>85% |
Active |
Native |
| 60007-F08H |
Ferret |
HEK293 Cells |
>95% |
|
C-His |
| 80234-R02H |
Rat |
HEK293 Cells |
>92% |
Active |
C-hFc |
| 90008-C08H |
Rhesus |
HEK293 Cells |
>90% |
Active |
C-His |
| 90008-CNAE |
Rhesus |
E. coli |
>90% |
Active |
Native |
| 10338-H02H |
IFNGR1 |
Human |
HEK293 Cells |
>95% |
|
C-hFc |
| 10338-H08H |
Human |
HEK293 Cells |
>97% |
Active |
C-His |
| 50705-M02H |
Mouse |
HEK293 Cells |
>95% |
Active |
C-hFc |
| 50705-M08H |
Mouse |
HEK293 Cells |
>98% |
Active |
C-His |
| 80182-R02H |
Rat |
HEK293 Cells |
>95% |
Active |
C-hFc |
| 90207-C02H |
Cynomolgus |
HEK293 Cells |
>95% |
Active |
C-hFc |
| 90207-C08H |
Cynomolgus |
HEK293 Cells |
>95% |
|
C-His |
| 50665-M08H |
IFNGR2 |
Mouse |
HEK293 Cells |
>97% |
|
C-His |
Other related proteins
- PD-1
- CD4
- IL-12
- PD-L1
- IL-18
- CD8
- IL-15
- IL-21
Antibodies for IFN-γ and Receptors
For use in ELISA, WB, FCM, and IHC, Sino Biological has created a panel of antibodies that target the IFN-γ cytokine and its receptors.
Anti-IFN-γ Antibody (FITC), Rabbit Mab Cat#: 11725-R003-F
Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.
Anti-IFN-γ Antibody, Rabbit PAb Cat#: 105908-T08
Immunochemical staining of human IFNG in human kidney cell. Image Credit: Sino Biological Inc.
Anti-IFNGR1 Antibody (APC), Mouse MAb Cat#: 10338-MM05-A
Flow cytometric analysis of Human IFNGR1 expression on human whole blood lymphocytes. Image Credit: Sino Biological Inc.