Host cell proteins: Detection and quantification

Host cell proteins (HCPs) are unwanted substances that can get into cell-based biotherapeutics, like recombinant proteins and antibodies during production. Chinese Hamster Ovarian (CHO) cells are commonly used to make these biotherapeutic products.

CHO cells naturally produce certain types of HCPs like PLBL2/PLBD2, PLA2G15, CLU, and LPL, which can harm the quality, effectiveness, and safety of biotherapeutics. Notably, HCPs can trigger unwanted immune reactions to the medicine. Because of this, there's a strong need for tests and substances to find and measure HCPs.

PLBL2/PLBD2 is a stubborn Host Cell Protein (HCP) present in CHO cells. Sino Biological, a world-renowned expert in recombinant technology, has recently created a special PLBL2/PLBD2 ELISA kit. This kit can precisely measure the amount of PLBL2/PLBD2 in Chinese hamster cells.

Sino Biological has also successfully produced antibodies and highly pure recombinant HCPs, which include PLBL2/PLBD2, PLA2G15, CLU, and LPL. These products make it easier to ensure the quality of biotherapeutics.

Sandwich ELISA for Detecting Host Cell Proteins. Image Credit: Sino Biological Inc.

Featured antibodies and ELISA kit for the detection of HCPs

PLBL2/PLBD2 ELISA kit, Chinese hamster: (Cat#: KIT5A7664)

Standard curve for PLBD2 quantification. Image Credit: Sino Biological Inc.

• Assay range: 390.63–25000 pg/mL
• Sensitivity: 60 pg/mL

Anti-PLBL2/PLBD2 antibody, Rabbit pAb: (Cat#: 13947-T24)

Immunochemical staining of human PLBL2/PLBD2 in human heart. Image Credit: Sino Biological Inc.

• Applications: ELISA, IHC-P

Anti-Clusterin antibody, Mouse mAb: (Cat#: 11297-MM01)

Immunofluorescence staining of human CLU in HeLa cells. Image Credit: Sino Biological Inc.

HeLa whole cell lysate probed with anti-CLU mouse mAb. Image Credit: Sino Biological Inc.

• Applications: ELISA, WB, FCM, ICC/IF

Featured recombinant HCPs

Recombinant PLBL2/PLBD2 protein, Chinese hamster: (Cat#: 5A7664-A08C) HPLC-verified

Image Credit: Sino Biological Inc.

• Purity: ≥ 95%

Recombinant LPL protein, Chinese hamster: (Cat#: 5A7668-A08E)

Image Credit: Sino Biological Inc.

• Purity: > 85%

More recommended products for HCPs (Partial list)

Source: Sino Biological Inc.

More antibodies against HCPs

• Anti-PLBL2/PLBD2
• Antibody Anti-LPL Antibody
• Antibody Anti-Clusterin