Gator® Plus: Quantitation, kinetics, and epitope binning through biolayer interferometry

The Gator^® Plus is a medium-throughput device that can be used for quantitation, kinetics, and epitope binning. This equipment, based on biolayer interferometry (BLI) technology, offers quick results.

Achieve more “hands-off” time and rise above the noise.

This fluidics-free instrument can hold 96-well or 384-well microplates. Rich datasets can be acquired with little hands-on time, with a walk-away time of more than six hours. Gator^® Plus is designed to analyze various biomolecules, including small molecules, antibodies, and proteins.

Gator^® Plus facilitates improved baseline stability, producing high-quality data for precise analysis of the most complex pairs. Gator^® Plus’s higher signal-to-noise ratio makes it simple to measure pM affinity pairs.

Specifications

Table 1. Source: Gator Bio, Inc.

General Specifications	Gator® Prime	Gator® Plus	Gator® Pro
Detection Technology	Next-gen Biolayer Interferometry
Simultaneous Reads	8	8	8, 16, 24, and 32
Spectrometers	8	8	32
Acquisition Rate	2, 5, and 10 Hz
Temperature Control	Ambient plus 4 °C to 40 °C
Dimension (HxWxD) and Weight	47 x 31 x 67 cm, 35 kg	68 x 44 x 73 cm, 55 kg	84 x 114 x 77 cm, 220 kg
Automation Compatible	No	No	Yes
Performance Specifications	Gator® Prime	Gator® Plus	Gator® Pro
Sample Types	Proteins, antibodies, peptides, nucleic acids, liposomes, viruses, small molecules
Maximum Sample Capacity	168	456	1152
Types of Analysis	Yes/no binding, quantitation, kinetics, affinity, off-rate ranking, epitope binning
Minimum Sample Volume	100 μL	40 μL	40 μL
Baseline Noise (RMS)	≤ 4 pm
Baseline Drift	≤ 0.12 nm/hour	≤ 0.1 nm/hour	≤ 0.1 nm/hour
Associate Rate kon	101 to 107 M-1 s-1
Dissociation Rate koff	10-6 to 10-1 s-1
Affinity Constant KD	10 pM to 1 mM
Binning Capacity	12x12	16x16	32x32

Gator^® Label-Free Analysis Systems. Image Credit: Gator Bio, Inc.

Biolayer interferometry (BLI)

Gator^® systems are label-free analysis tools that use next-generation biolayer interferometry (BLI) technology. Biosensing probes are immersed in samples to identify biomolecular interactions.

Gator^® probes are micro glass rods with specialized optical layers and surface chemistries coated on the distal ends.

The association or disassociation of biomolecules causes a phase shift in the optical interference pattern produced by the sensing surface of a probe. Continuous phase-shift measurements yield binding curves.

The sensorgram shows the real-time association and disassociation curves for a binding kinetics experiment using a Gator^® system. Image Credit: Gator Bio, Inc.

Gator^® systems’ simplicity, versatility, flexibility, and throughput have facilitated numerous therapeutic development, manufacturing, and life science research applications.

A powerful tool for discovery, development, and manufacturing

The next-generation BLI is more sensitive and has a more robust performance than other commercial BLI products. It also supports various applications, from drug discovery to therapeutic manufacturing.

Biotherapeutics

• Kinetics analysis
• Antibody titer measurements
• Epitope binning
• Manufacturing QC
• Process development
• Pharmacokinetics

Gene therapy

• Receptor interaction
• AAV quantitation and kinetics
• Neutralizing/total antibody detection
• Gene expression

Drug discovery and development

• Protein–small molecule interaction
• Peptide binding analysis

Life science research

• Receptor–ligand binding
• Protein-protein interaction
• Assay development and optimization

A user-friendly label-free technology

Gator^® Systems are made up of instruments, probes, and a software package for data acquisition and analysis.

• Simple and fast assay setup
• Kinetics and affinity analysis
• Automated quantitation
• Quantitation, kinetics, and regeneration in one run
• Assay template generation
• Real-time binding curves
• Epitope binning
• Report generation

Gator^® software for GMP and GLP

Users in GMP or GLP environments can use Gator^® Part11 Software to abide by FDA 21 CFR Part 11 regulations. The Part11 Software collects data that is time-stamped and traceable. Account management, improved audit trails, and documented user sessions are all in accordance with FDA guidelines.

A full suite of applications

Table 2. Source: Gator Bio, Inc.