In recent years, non-destructive 3D imaging techniques such as micro-computed tomography (microCT) have increasingly captured the attention of preclinical researchers across almost every discipline. For angiography, microCT provides volumetric data at high resolution, but the technique’s inherent X-ray attenuation means that vasculature cannot be distinguished from surrounding soft tissues.
In this webinar, Dr. Ruslan Hlushchuk (Universität Bern Institute of Anatomy) and Dr. Kjell Laperre (Bruker Biospin’s Pre-Clinical Imaging Division) will introduce a novel radiopaque polymerizing contrast agent called µAngiofil that can be used to overcome this challenge.
For ex vivo angiography, µAngiofil enables resolution down to the fine-capillary level. In addition, samples can be used for subsequent morphological and quantitative analysis without extensive post-processing.
Ruslan will describe the microCT-based visualization that can be achieved with µAngiofil and the perfusion procedure, visual controls and sample harvesting and handling involved. Bruker Biospin’s Skyscanner 1272 will be introduced, as well as the higher energy Skyscanners 1273 and 2214 that enable the imaging of denser samples.
This webinar will interest facility managers, contract research organizations and preclinical researchers who specialize in cardiovascular-, neurovascular-, implantology- and angiogenesis-related fields.
Key topics that will be discussed in this webinar include:
- The perfusion procedure/protocol for µAngiofil – visual control of the perfusion quality, sample harvesting and tendering.
- The excellent, non-destructive imaging of microvasculature down to the finest capillaries, as well as in the presence of bone tissue and/or metal implants.
- The potential for correlative morphology (facilitated by the autofluorescence of the µAngiofil).
- Examples of quantitative analysis
- Bruker BioSpin's high resolution desktop micro-CT, Skyscan 1272.
Speakers
Ruslan Hlushchuk, PhD
Lecturer and Group Leader of Topographic and Clinical Anatomy, Universität Bern Institute of Anatomy
Dr. Ruslan Hlushchuk, Lecturer and Group Leader of Topographic and Clinical Anatomy at the Universität Bern Institute of Anatomy. Head of the micro-CT group, Ruslan specializes in (micro)vascular imaging and microangioCT.
Kjell Laperre, PhD
Micro-CT Market Product & Applications Manager, Bruker Biospin, Pre-Clinical Imaging Division