Being one of the sequencers with the highest speed among mid-low throughput, DNBSEQ-G99 is advanced depending on MGI’s core DNBSEQ sequencing technology, backed by innovations in biochemistry, fluidics, temperature control, optics, and other core systems. DNBSEQ-G99 is relevant for numerous sequencing methods; it requires 12 hours to finish PE150 sequencing.
Features
- 8~48 GB per run
- Aids multiple read lengths, such as PE50, SE100, PE150
- Just 12 hrs for PE150 (from loading to FASTQ)
- Suitable for several sequencing techniques
Image Credit: MGI
Performance parameter
Table 1. Source: MGI
| Maximum number of Flow cells |
Lanes/Flow cell |
Effective Reads*/Flow Cell |
Supported Reads Lengths**** |
Data Output |
Q30** |
Run Time |
| 2 |
1 |
80M |
SE100/PE50 |
8-16 G |
>90% |
5 h |
| PE150 |
24-48 G |
>85% |
12 h |
| APP-C SE100*** |
8-16 G |
>90% |
5 h |
| APP-C PE150 |
24-48 G |
>85% |
12 h |
* The effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method.
** The percentage of bases above Q30 is the average of an internal standard library over the entire run. Actual performance is affected by factors such as sample type, library quality, and insert fragment length.
*** Estimated release in 2023 Q1.
**** DNBSEQ-G99 also supports SE50 and PE100 sequencing, and the existing kits can support SE50, PE100 read length sequencing.
Table 2. Source: MGI
| Method |
Application |
Recommended read length |
Data size |
Sample per Run |
| 1 flow cell |
2 flow cells |
| Targeted Capture/ Multiplex PCR |
Oncology panel |
PE150 |
Small panel: ~1 Gb/Sample |
24 |
48 |
| Genetic disease diagnosis (small panel) |
PE150 |
Thalassemia: ~0.2 M/Sample
Deafiness: ~5 Gb/Sample |
4 |
8 |
| ATOPlex panel |
PE100, PE150 |
Respiratory tract panel: 5 M/Sample
COVID-19 panel: 5 M/Sample |
16 |
32 |
| WES |
PE150 |
15 Gb/Sample |
1-2 |
2-4 |
| Methylation |
Oncology targeted methylation panel |
PE150 |
~5 Gb/Sample |
4 |
8 |
| Small Genome Sequencing |
Metagenomics for pathogen detection |
SE50, SE100 |
Meta: 20 M/Sample |
4 |
8 |
| Microbial WGS |
PE100, PE150 |
Single bacterium: ~1 Gb/Sample |
16-24 |
32-48 |
| LOW pass whole-genome sequencing |
NIPT |
SE35 |
~10 M/Sample |
8 |
16 |
| PGS |
SE35 |
| Transcriptome sequencing |
RNA-Seq |
SE50, PE100, PE150 |
Expression profiling: ~1 Gb/Sample
Transcriptome: ~8 Gb/Sample |
2-4 |
4-8 |
*Unless otherwise informed, StandardMPS and CoolMPS sequencing reagents, and sequencers for use with such reagents, are not available in Germany, Spain, UK, Sweden, Belgium, Italy, Finland, Czech Republic, Switzerland, Portugal, Austria, and Romania. Unless otherwise informed, StandardMPS sequencing reagents, and sequencers for use with such reagents are not available in Hong Kong.
*Products are provided for Research Use Only. Not for use in diagnostic procedures (except as specifically noted).