Microsaic’s patented MiD products and accompanying software deliver quick insights into biological or chemical samples and reactions using miniaturized chip-based technologies.
Microsaic 4500 MiD®
Video Credit: Microsaic Systems plc
The vacuum system, electronics, and computer are all housed inside the 4500 MiD®. It can be installed in places where no other mass spectrometer can be simply deployed, all while maintaining the performance of a normal mass spectrometer system.
With the inclusion of the Microsaic MiDas™ compact interface sampling module, the 4500 MiD® can now be used in a wide range of on-line, at-line, and off-line applications.
- Small footprint allows deployment anywhere inside the laboratory or processing facilities
- Low maintenance costs with a completely tool-free front-end
- A wholly integrated and sophisticated system, with no external vacuum pumps, and no external PC
- User-friendly, easy “plug and play” consumables
Chip-based technology
The 4500 MiD® is a reliable, robust, and user-friendly device that features Microsaic’s three main patented chip-based technologies, the ionchip®, spraychip®, and vac-chip™, as well as increased mass range, a entirely tool-free front-end for improved control, an inventive and robust user interface, and improved electronics. A change in front-end alignment has also improved system uptime while lowering contaminant deposition downstream.
- Vac-ch¡p™
- Ionchip®
- Spraychip®
Mass range
Improving the mass range to 1400 m/z enables new applications such as peptide analysis using Nano LC-MS, which is appropriate for the 4500 MiD®’s exceptionally effective ESI source and a strong propensity for multiple charging.
The Microsaic 4500 MiD® is simple to operate and maintain, and it also consumes less solvent, nitrogen, and power, lowering operating expenses and providing a greener alternative for mass detection.
Table 1. Technical Spec. Source: Microsaic Systems plc
| Technical Spec |
|
| Dimensions |
55 x 35 x 25 cm (22 x 14 x 10 in.) (including PC, pump and exhaust) |
| Weight |
32 kg (including PC, pump and exhaust) |
| Ion Source |
spraychip® – electrospray ionization source |
| Ionization Modes |
Positive and negative ESI |
| Sample Inlet |
Compatible with standard capillaries and unions e.g., 1/32” tubing |
| Flow Rate Range |
0.3-2000 µL/min |
| Mass Analyzer |
ionchip® – quadrupole mass filter |
| Mass Range |
50-1400 m/z |
| Mass Accuracy |
± 0.3 m/z in full scan* |
| Mass Resolution |
0.7 m/z ± 0.1 @ FWHM* |
| Sensitivity |
10pg of reserpine yields a peak in SIM mode with a S/N ratio of 10:1 (RMS) |
| Interface |
vac-chip™ – off-axis microengineered atmospheric pressure interface |
| Detector |
Electron multiplier |
| Dynamic Range |
3-4 orders of magnitude |
| Scan Modes |
Full scan, SIM (8m/z channels), simultaneous scan/SIM, and timed SIM |
| Scan Rate |
m/z 1,500/s (7,500 pts/s) |
| Pumping System |
Integrated oil-free pumps (no floor pump needed) |
| Software Control |
Masscape® Clarity, PrepCon, Remote Operations Protocol |
| Connections |
Ethernet, 5 x USB
2 x digital in (e.g., LC trigger input), 2 x digital out
4 x in (e.g.; UV Trace), 4 x out (e.g., triggering external device)
1 x RS232 (e.g., syringe pump control) |
Table 2. Utility requirements. Source: Microsaic Systems plc
| Utility requirements |
|
| Power Requirements |
Power: 250W (operating), 300 W (peak)
Line voltage: 100-240 V AC±10%
Line frequency: 50/60 Hz |
| Nitrogen Gas |
Pressure 2-6 bar (29-87 psi)
Purity (≥99,5%)
6 mm OD push fit connection |
| Pump Exhaust |
Exhaust connects to an 8 mm OD push fit |