CD16, CD32, CD64, and usage in antibody therapy

FCγ receptors (FCγRII/CD32, FCγRIII/CD16, and FCγRI/CD64) are known as cellular receptors for IgG that add up to immune complex clearance, antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and comprise several subtypes.

FCγR polymorphisms can potentially forecast responses to immunotherapies, thereby directing personalized treatment methods. As far as therapeutic antibody development is concerned, the efficacy of mAbs is identified by their affinity to FCγRs, which impacts their engagement with immune effector cells.

FcγR-binding is vital for developing immune checkpoint antibodies against CTLA-4, PD-1, TIM-3, PD-L1, LAG3, CD73, TIGIT, VISTA, and B7-H3. Interactions of mAb with FcγRs also affect cytokine release, which is vital in the immune response. Moreover, FCγRs can serve as biomarkers to monitor treatment responses and are studied in combination therapies such as HER2-targeted mAb therapy. 

Learn More About FCγ Receptors

As a leading bioreagents company, Sino Biological offers high-purity and bioactivity-validated FcγR and other-related products to assist the development of new antibody therapies.

Table 1. The types and characteristics of FcγRs. Source: Sino Biological US Inc

Name	FcγRI/CD64	FcγRIIA/CD32a	FcγRIIB/CD32b	FcγRIIC/CD32c	FcγRIIIA/CD16a	FcγRIIIB/CD16b
Structure
Function	Activating	Activating	Inhibitory	Activating	Activating	Activating
Affinity	High	Low	Low	Low	Low	Low
Effect of Antibody Binding	ADCP, cytokine release	ADCC, ADCP, vaccinal effect	Inhibits ADCC, ADCP, B cell activation	Enhances ADCC, ADCP, B cell activation	ADCC, ADCP	Decoy receptor that inhibits ADCP

Featured recombinant FcγR proteins

Sino Biological has developed a panel of high-quality FcγR proteins from several species to assist in developing antibody therapies.

• High-Purity
• Activity-Validated
• HPLC-Verified
• Cited by scientists
• Validated by BLI Assay

Human CD16a protein (His & AVI Tag)

(Cat#: 10389-H27H1-B)

BLI assay: Loaded biotinylated human CD16a protein on SA Biosensor can bind Bevacizumab (IgG1). Image Credit: Sino Biological US Inc

ELISA functional assay: Immobilized biotinylated human CD16a protein can bind human IgG1 (Cat#: 10702-HNAC). Human CD32a Protein (His & AVI Tag) (Cat#: 10374-H27H-B) HPLC-verified 9 Citations. Image Credit: Sino Biological US Inc

Human CD32a protein (His & AVI Tag)

(Cat#: 10374-H27H-B)

ELISA functional assay: Immobilized human IgG1 at can bind human biotinylated CD32a protein. Image Credit: Sino Biological US Inc

Human CD32b protein (His & AVI tag)

(Cat#: 10259-H27H-B)

BLI assay: Loaded biotinylated human CD32b/c protein on SA Biosensor can bind Bevacizumab (IgG1). Image Credit: Sino Biological US Inc

Human CD64 protein (His & AVI tag)

(Cat#: 10256-H27H-B)

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated human CD64 protein. Image Credit: Sino Biological US Inc

Mouse CD64 protein (His & AVI tag)

(Cat#: 50086-M27H-B)

ELISA functional assay: Immobilized Rituximab (IgG1) can bind biotinylated mouse CD64 protein. Image Credit: Sino Biological US Inc

More recombinant FcγR proteins (Partial)

Table 2. Source: Sino Biological US Inc

Featured antibodies for FcγRs

A panel of antibodies for FcγRs and also related molecules with applications in IHC-P, FCM, ELISA, and WB has been developed by Sino Biological.

Anti-CD32a, mouse mAb

(Cat#: 10374-MM02-F)

Flow cytometric analysis of human CD32a expression on human whole blood lymphocytes (left panel), monocytes (middle panel), and granulocytes (right panel). Image Credit: Sino Biological US Inc

Anti-CD16a, rabbit pAb

(Cat#: 10389-T24)

Applications: ELISA, IHC-P

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody. Image Credit: Sino Biological US Inc

Anti-CD32b, rabbit mAb

(Cat#: 10259-R110)

Applications: WB, IHC-P

Daudi Whole Cell (A) and THP1 Whole Cell (B) Lysate probed with anti-CD32b rabbit monoclonal antibody. Image Credit: Sino Biological US Inc

Anti-CD64, rabbit mAb

(Cat#: 50086-R027)

Applications: FCM, ICC/IF

Immunochemical staining CD16a in human spleen with rabbit polyclonal antibody. Image Credit: Sino Biological US Inc

More antibodies for FcγRs (Partial)

Table 3. Source: Sino Biological US Inc